By Thomas Scheper, P. Bajpai, P.K. Bajpai, D. Dochain, N.N. Dutta, A.C. Ghosh, R.K. Mathur, A. Mukhopadhyay, M. Perrier, P.L. Rogers, H.S. Shin, B. Wang
This quantity offers with such themes as realities and tendencies in enzymatic prebleaching of Kraft pulp, biotransformation for l-ephedrine creation, inclusion our bodies and purification of proteins in biologically energetic types, and extraction and purification of cephalosporin antibiotics.
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Extra info for Biotreatment, Downstream Processing and Modelling (Advances in Biochemical Engineering Biotechnology)
3 Biotransformation Using Y e a s t s - F r e e Cells . . . . . . . . . . . . . . . 1 Fed-Batch Process . . . . . . . . . . . . . . . . . . . . . 2 Continuous Process . . . . . . . . . . . . . . . . . . . . . 4 Biotransformation Using Y e a s t s - I m m o b i l i s e d Cells . . . . . . . . . . . . 5 Biotransformation Using Purified P D C Free Enzyme . . . . . . . . . . . . 1 Characteristics of Purified P D C .
With L-PAC production, both the substrate (benzaldehyde) and the product (L-PAC) have significant inhibitory effects on yeast metabolism and PDC activity. These factors necessitate the design of a more complex continuous culture process, and suggest one involving multistage operation and later stage benzaldehyde feeding in order to sustain steady-state conditions. Other workers have reported on the use of a single-stage continuous culture for the production of active yeast cells for L-PAC production , however the biotransformation of benzaldehyde was carried out in batch culture.
Xylanases are easily applied and require essentially no capital expenditure. Because chlorine dioxide charges can be reduced, xylanase may help eliminate the need for increased chlorine dioxide generation capacity. Similarly, the installation of expensive oxygen delignification facilities may be avoided. The benefit of a xylanase bleach-boosting stage can also be taken to shift the degree of substitution towards higher chlorine dioxide levels while maintaining the total dosage of active chlorine.