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Capillary Electrophoresis: Instrumentation and Operation by Wim Kok

By Wim Kok

In this quantity the overall rules of capillary (zone) electrophoresis, the functionality of this separation approach to be anticipated, the instrumentation and strategies to be had and functional directions for the operaton of CE tools may be mentioned. The emphasis may be at the regimen program of CE with commercially on hand tools, yet realization may also be paid to devolpments nonetheless in a examine level and their attainable implications for the future.

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Approximate analytical expressions can be The Background Electrolyte References [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [II] [12] E Kohlrausch, Ann. Phys. , 62 (1897) 14. YP. Dole, 1. Am. Chern. , 67 (1945) 1119. G. Longworth, J. Phys. M. Hjelmeland and A. Crambach, Electrophoresis, 3 (1982) 9. S. Hjerten, in G. ), Topics in Bioelectrochemistry and Bioenergetics, Vol. 2, Wiley, New York, p. 106. P. Mikkers, EM. M. Verheggen, J. , 169 (1979) 1. T. A. Hartwick, J. , 589 (1992) 307. Y Sustacek, E Foret, and P.

S. Hjerten, in G. ), Topics in Bioelectrochemistry and Bioenergetics, Vol. 2, Wiley, New York, p. 106. P. Mikkers, EM. M. Verheggen, J. , 169 (1979) 1. T. A. Hartwick, J. , 589 (1992) 307. Y Sustacek, E Foret, and P. Bocek, J. , 545 (1991) 239. L. Beckers, J. Chromatogr. A, 693 (1995) 347. X. Xu, W. Th. Kok and H. Poppe, J. Chromatogr. A, 742 (1996) 211. S. Y. Zhukov, Electrophoresis, 13 (1992) 838. G. Righetti, J. Chromatogr. A, 667 (1994) 257. 1 Hydrodynamic and electrokinetic injection Basically there are two different methods to introduce the sample into the first small part of the capillary: hydrodynamic (driven by a pressure difference over the capillary) and electrokinetic.

Flee 7 C [10. 15 Calculated influence of an external radial field on the electroosmotic mobilit~. Ionic strength 10 mmol L-I; Pco" = 0 (a), 30 (b) and 60 (c) x 10- m 2 V 1 S·l Reproduced from ref [8]. S-34 In 1990 Lee and coworkers [30] found a way to change the EOF, and even to reverse its direction, by applying an external radial electric field. , [31, 32]). Basically, the technique of external EOF control consists of the application of a high voltage radially over the wall of the capillary, in addition to the longitudinal field for the separation.

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