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Exp. Med. 131, 711 (1970). , J. Exp. Med. 137, 967(1973). , J. Immunol. 104, 128 (1970). S. 411. Academic Press, New York, 1975. S. 281. Academic Press, New York, 1975. S. 261. Academic Press, New York, 1975. , Science 176, 1432 (1972). Immunol. 115, 1135 (1975). , J. Immunol. 115, 440 (1975). , J. Exp. Med. 141, 138 (1975). S. 391. Academic Press, New York, 1975. 32 25. 26. 27. 28. 29. 30. 31. 32. 33· 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. DAVID F. P. Eijsvoogel, D. P.

P. 297. Academic Press, New York, 1976. , J. Immunol. 113, 151 (1974). B. 6. American Elsevier, New York, 1973. DISCUSSION Kaplan, Ottawa: In the experiments with the effects of colchicine on the MLR, it was quite interesting that this drug did not inhibit the concanavalin A response but it did inhibit stimulation of the MLR. Did you separately treat stimulator cells and responder cells with colchicine to see if the effect was selectively on stimulation or on response? Ranney: We did some initial studies on responder cells but did not concentrate on that system because we expected a significant inhibitory effect of colchicine on the responder cell.

PROTEINS -* I — I — * I I *\ 1 MICROTUBULE POLYMERIZATION COLCHICINE V INBLAST INE VINCRISTINE Fig. 4. Schematic diagram of metabolic coupling between the intracellular processes (enclosed by boxes) whose pharmacologic inhibition prevents allogeneic stimulator cell activity. Π QJ 3 CD -i -σ CD X (/) z —· o -< —· o * —· XJ 3 Q. to • -< —· —· —· < —· —· —·*< —· —· —· —· —· —*< —· —· <1 o < 3 O. CD — "1 · CO QJ 3 O. CD ~\ «· ——· —· —· CD c —· z O O CO O r t Pt QJ c cr cr —· c rt 3" N CD 1 QJ CD CD a.

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