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Continuous Cultivation of Microorganisms. Proceedings of the by I. Malek

By I. Malek

Non-stop Cultivation of Microorganisms

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Extra info for Continuous Cultivation of Microorganisms. Proceedings of the Second Symposium held in Prague June 18–23, 1962

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This applies also to the question of output. The output of cells from a multi-stream system is given by ¿ ÷ , where x is the concentration of cells issuing from the last fermenter and D the average dilution rate, defined as the sum of all the inflows divided by the sum of all the fermenter volumes. The output therefore depends upon a greater number of independent variables than that of a single-stream system, and cannot be expressed by any simple formula. However, it is not too difficult to see that: (i) the output of a multi-stream system may, according to the ratios of the inputs to the different stages, be either greater or less than that of a single-stage system of the same total volume and operated at the same flow rate; (ii) the maximum possible output of a multi-stream system may equal (though not exceed) that of a one-stage system.

Herber t an d Dr . Powell two questions : Accordin g to our reserc h of alcoholi c an d acetone-butano l fermentatio n wit h simultaneousl y recyclin g of th e microbia l cultur e fro m th e end fermentor s int o th e mai n fermentor , infectio n of th e fermentin g mediu m alway s occurred , sometime s even stoppin g th e fermentatio n process . D o you thin k tha t thi s exampl e shoul d not b e used in your mathematica l generalisation s ? I n th e extrem e case thes e generalisation s mus t be limite d to certai n medi a or processe s which ar e insensitiv e to infection .

W e ha d ourselve s discusse d variou s method s for approximatin g t o a tubula r fermenter , I ha d reache d simila r conclusion s as t o ho w to do it, thoug h we never got aroun d to buildin g one. Wit h regar d t o th e proble m of aeratio n of tubula r fermenters , one can thin k of variou s ways in which thi s could be done , thoug h mor e easily in th e factor y tha n in th e laboratory . On e could , for example , use a meta l tub e an d ru n it unde r pressure , introducin g pur e oxygen at a numbe r of point s alon g th e tube .

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