Engineering

Genetic Engineering Techniques: Recent Developments by P. C. Huang

By P. C. Huang

Genetic Engineering thoughts: fresh Developments

summary: Genetic Engineering strategies: contemporary advancements

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DNA was digested with PstI or Hpal and the fragments were methylated in vitro with EcoK and ^H-Ado-Met. The samples were then electrophoresed in 1% agarose gels. Samples 1 and 6 were stained with ethidium bromide and the other samples were subjected to fluorography. Samples 1 and 6 are the products of PstI and Hpal digestion. Samples 2,3,7 and 8 contain unmodified DNA, ^H-AdoMet and EcoK (samples 2 and 7 have ATP, and 3 and 8 have the imido analog of ATP). Samples 4, 5, 9 and 10 have heteroduplex DNA, 3H-AdoMet and EcoK (4 and 9 have ATP, 5 and 10 have the imido analog).

Natl. Acad. Sei. USA 76:200. C. (1980). Trends in Biochem. , p. 219. C (1981). D. Boyer, e d . ) , Vol. , p. 331. Academic Press, New York. C (1982a). In "Nucleases" (R. Roberts and S. ), Cold Spring Harbor Laboratory, New York, in press. C. (1982b). In "The Future of Nucleic Acids" a symposium volume of the first Asian Molecular Biology Organization Meeting, Kyoto, Japan, 1981. In press. C and Kirkegaard, K. (1981). In "Gene Amplification and Analysis, Vol. S. ), p. 456. Elsevier/North-Holland, New York.

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