Molecular Markers in Plants by Robert J. Henry

By Robert J. Henry

Molecular Markers in Plants surveys an array of applied sciences utilized in the molecular research of crops. The position molecular markers play in plant development has grown considerably as DNA sequencing and high-throughput applied sciences have matured. This well timed assessment of applied sciences and methods will offer readers with an invaluable source at the most recent molecular technologies.

Molecular Markers in Plants not just reports previous achievements, but in addition catalogs contemporary advances and appears ahead in the direction of the long run program of molecular applied sciences in plant development. starting chapters examine the improvement of molecular applied sciences. next chapters examine a variety of purposes for using those advances in fields as different as plant breeding, construction, biosecurity, and conservation. the ultimate chapters glance ahead towards destiny advancements within the field.

Looking largely on the box of molecular applied sciences, Molecular Markers in Plants could be a necessary addition to the library of each researcher, establishment, and corporate operating within the box of plant improvement.

Chapter 1 Evolution of DNA Marker know-how in crops (pages 1–19): Robert J. Henry
Chapter 2 Whole?Genome Sequencing for Marker Discovery (pages 21–34): Mark Edwards
Chapter three Amplicon Sequencing for Marker Discovery (pages 35–56): Timothy R. Sexton and Frances M. Shapter
Chapter four Transcriptome Sequencing for Marker Discovery (pages 57–66): Susan Gillies
Chapter five Molecular Markers in Plant development (pages 67–80): Peter J. Prentis, Edward okay. Gilding, Ana Pavasovic, Celine H. Frere and Ian D. Godwin
Chapter 6 purposes of Molecular Markers in Plant Conservation (pages 81–98): Maurizio Rossetto and Paul D. Rymer
Chapter 7 Molecular Markers for Plant Biosecurity (pages 99–117): Andrew D. W. Geering
Chapter eight Molecular Markers for Harnessing Heterosis (pages 119–136): Gopala S. Krishnan, A. ok. Singh, Daniel L. E. Waters and Robert J. Henry
Chapter nine Genetic variation Discovery and Its Use in Genome Characterization of Agronomically vital Crop Species (pages 137–167): Stephane Deschamps and Matthew A. Campbell
Chapter 10 destiny clients of Molecular Markers in crops (pages 169–190): Reyazul R. Mir and Rajeev okay. Varshney

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Most of these problems can be resolved by higher coverage. As for Sanger sequencing, the error rate increases with the position in the sequence, this is caused by a reduction in enzyme efficiency or loss of enzymes (resulting in a reduction of the signal intensities), some molecules no longer being elongated. 5%. , 2009). A limitation of the 454 sequencing is to properly interpret long stretches (>6) of the same nucleotide (homopolymer DNA segments); though substitution errors are rarely encountered.

Turner, S. and Kasarskis, A. (2010) A window into third-generation sequencing. Human Molecular Genetics, 19, R227–R240. Shendure, J. L. (2008) Next-generation DNA sequencing. Nature Biotechnology, 26, 1135–1145. B. et al. (2005) Accurate multiplex polony sequencing of an evolved bacterial genome. Science, 309, 1728–1732. BLBS114-c03 BLBS114-Henry 3 Trim: 244mm×172mm September 10, 2012 8:56 Amplicon Sequencing for Marker Discovery Timothy R. Sexton and Frances M. Shapter Contents Introduction 36 Background 36 Maximizing Efficiency Through Sample Pooling 38 Limitations of Amplicon-Based MPS 44 Bioinformatics 51 Concluding Remarks 52 Acknowledgments 52 References 53 Molecular Markers in Plants, First Edition.

McDonald, S. et al. (2011) Genome Diversity in Wild Grasses under Environmental Stress. Proceeding of the National Academy of Sciences of the United States of America, 108, 21139–21144. , Blakeney, A. et al. (2000) PCR based molecular marker for the fragrance gene in rice (Oryza sativa L). Theoretical & Applied Genetics, 101, 364–371. , Furtado, A. J. (2012) Gene expression in the developing aleurone and starchy endosperm of wheat. Plant Biotechnology Journal, 10, 668–679. , Mayer, P. J. (1994) A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis.

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