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New Techniques in Nutritional Research by Roger G. Whitehead, Roger Whitehead

By Roger G. Whitehead, Roger Whitehead

New innovations in dietary learn includes the court cases of the 9th Annual Bristol-Myers Squibb/Mead Johnson Symposium on nutrients examine hung on September 11-13, 1989 in Cambridge, England. The papers spotlight a couple of novel suggestions which are at the moment utilized in dietary examine, together with the doubly categorised water strategy; the categorised bicarbonate approach; X-ray computed tomography; neutron activation research; magnetic resonance imaging; and the appliance of solid isotope tracers.
Divided into 4 sections encompassing 20 chapters, this quantity starts off with an summary of sturdy isotopes and their functions in food technological know-how, together with diet study, and within the research of mineral metabolism. It then discusses sleek methods for measuring strength expenditure and physique composition, classical and glossy equipment of anthropometry, size of bone mineral content material in vivo, and imaging innovations in research of bone prestige.
Nutritional scientists will locate this e-book an invaluable resource of knowledge with regards to the sphere.

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New Techniques in Nutritional Research

New ideas in dietary study includes the lawsuits of the 9th Annual Bristol-Myers Squibb/Mead Johnson Symposium on foodstuff examine hung on September 11-13, 1989 in Cambridge, England. The papers spotlight a couple of novel thoughts which are at the moment utilized in dietary examine, together with the doubly categorized water approach; the categorised bicarbonate procedure; X-ray computed tomography; neutron activation research; magnetic resonance imaging; and the appliance of strong isotope tracers.

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Our purpose here, however, is to mention this particular application of N tracers, since it is an example of a specific nutritional problem that is well explored through use of stable isotope probes. , Wu, 1951; Wu and Sendroy, 1959). However, a few years ago, we carried out a study in rats to explore whether ammonia served as a significant nitrogen precursor for nitrate synthesis (Wagner et al, 1985). Hence, merely for purposes of emphasizing use of N in nutritional-metabolic research, this investigation provided estimates of ammonia flux, which amounted to 6 //moles/hr/kg body weight in adult male rats.

Taylor, P. W. Watt, and W. M. Bennet change of insulin availability (Watt et al, 1989; Bennet et al, 1990a). In studies in anesthetized pigs, the same effects may be obtained when insulin secretion is abolished by somatostatin and insulin is replaced to a low physiological concentration (P. W. Watt and M. J. Rennie, unpub­ lished results). Furthermore, we think we have provided an answer to a conundrum that has been bothering a number of us for some years; that is, why have we been unable to measure any stimulatory effect of insulin on muscle protein synthesis in humans (Bennet et al, 1990b; Gelfand and Barrett, 1987; Pacy et al, 1987)?

J. 13 2 5 Clin. Nutr. 35, 1 0 3 3 - 1 0 4 0 . Waterlow, J. C , and Stephen, J. M. L. (1981). " Applied Science, London. Waterlow, J. C , Garlick, P. , and Millward, D. J. (1978). , Amsterdam. Watt, P. , Stenhouse, M. , Corbett, Μ. , and Rennie, M. J. (1989). tRNA charging in pig muscle measured by [l- C]-Leucine during fasting and infusion of amino acids. Clin. Nutr. 8, 0 . 7 4 . 13 This page intentionally left blank Protein and Amino Acid Turnover Using the Stable Isotopes N, C, and H as Probes 15 13 2 Vernon R.

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