Engineering

Tissue engineering and artificial organs by Joseph D Bronzino

By Joseph D Bronzino

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Coli. Let us also presume that the chosen plasmid includes a gene for survival in the presence of the antibiotic ampicillin. Such a survival gene will provide a means for screening for and isolating a successful transformant when manipulated cells are grown in ampicillin-containing growth medium. 8, the first step is to amplify the source of human insulin DNA. Using PCR and an appropriate primer design, the DNA encoding for insulin is amplified and flanked with restriction sites of our choosing.

7 Engineering the System. . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Introduction Bacteria are unicellular, relatively simple, and can double in very short times. These attributes can be exploited for technological advantage. They are useful for the expression of large quantities of products, such as proteins and enzymes, as well as small molecules that are not efficiently synthesized in the laboratory via bio-organic chemistry.

7 Engineering the System. . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Introduction Bacteria are unicellular, relatively simple, and can double in very short times. These attributes can be exploited for technological advantage. They are useful for the expression of large quantities of products, such as proteins and enzymes, as well as small molecules that are not efficiently synthesized in the laboratory via bio-organic chemistry.

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